Recombinant human FIH-1/HIF-1AN protein
Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1alpha C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1alpha transactivation domain results in blockage of the HIF1alpha-p300/CBP interaction and represses transcriptional activity of HIF1alpha-targeted genes. Recombinant human HIF1AN protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
40.2 kDa (349aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Fukuba H., et al. (2008) Neurosci Lett. 433 (3) :209-14.; ; Li J., et al. (2007) Mol Cell Biol. 27 (20) :7345-53.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0)
Scientific Category
Cell Cycle
NCBI Accession Number
NP_060372
Uniprot Accession Number
Q9NWT6
Species
Human
AA Sequence
Frequently Asked Questions
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