Recombinant human FIH-1/HIF-1AN protein

Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1alpha C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1alpha transactivation domain results in blockage of the HIF1alpha-p300/CBP interaction and represses transcriptional activity of HIF1alpha-targeted genes. Recombinant human HIF1AN protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hypoxia-inducible factor 1 alpha subunit inhibitor, Hypoxia-inducible factor asparagine hydroxylase, Factor inhibiting HIF-1, FIH1, Hypoxia-inducible factor 1, alpha subunit inhibitor Hypoxia inducible factor asparagine hydroxylase, Factor inhibiting HIF1, FIH 1, FLJ20615, FLJ22027, Hypoxia inducible factor 1 alpha inhibitor, Hypoxia inducible factor 1 alpha subunit inhibitor, Peptide aspartate beta dioxygenase, DKFZp762F1811, Hypoxia-Inducible Factor-1 Alpha Inhibitor

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

40.2 kDa (349aa) confirmed by MALDI-TOF

Additionnal Information

FIH1, HIF1AN, HIF0904-10 µg, HIF0904-20 µg, HIF0904-50 µg, HIF0904-100 µg, HIF0904-250 µg, HIF0904-500 µg, HIF0904-1 mg, HIF0904-10, HIF0904-20, HIF0904-50, HIF0904-100, HIF0904-250, HIF0904-500, HIF0904-1

References & Citations

Fukuba H., et al. (2008) Neurosci Lett. 433 (3) :209-14.; ; Li J., et al. (2007) Mol Cell Biol. 27 (20) :7345-53.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0)

Scientific Category

Cell Cycle

NCBI Accession Number

NP_060372

Uniprot Accession Number

Q9NWT6

Species

Human

AA Sequence

MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN