Recombinant human HIF-1 alpha/HIF1A protein

Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (Hif-1alpha, Hif-2 or Hif-3) . The activation of Hif-1 is closely associated with a variety of tumors and oncogenic pathways. Hif-1alpha consists of DNA binding domain (DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Hif-1 alpha (530-826 residues) contains two TAD and inhibitory domain. Recombinant Hif-1 alpha (530-826 residues) was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hypoxia inducible factor 1 subunit alpha, Hypoxia-inducible factor 1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, PASD8, MOP1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.8 kDa (298aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

HIF1 a, Hypoxia-inducible factor-1a, HIF1A, MOP1, Hif-1 alpha (530-826 aa), HIF-1 alpha, HIF1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, Hypoxia-inducible factor 1 alpha, Hypoxia-inducible factor-1a Basic helix loop helix PAS protein MOP1, ARNT interacting protein, bHLHe78, HIF 1A, HIF 1alpha, HIF1 A, Hypoxia inducible factor 1 alpha, Hypoxia inducible factor 1 alpha isoform I.3, Hypoxia inducible factor 1 alpha subunit, Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor, Member of PAS protein 1, Member of PAS superfamily 1, Member of the PAS Superfamily 1, MOP 1, PASD 8, PASD8, HIF0501-10 µg, HIF0501-20 µg, HIF0501-50 µg, HIF0501-100 µg, HIF0501-250 µg, HIF0501-500 µg, HIF0501-1 mg, HIF0501-10, HIF0501-20, HIF0501-50, HIF0501-100, HIF0501-250, HIF0501-500, HIF0501-1

References & Citations

Okuyama H. et al., (2006) J. Biol Chem. 281 (22) :15554-63; ; Berra E. et al., (2006) EMBO.7 (1) :41-5;

Other References

Hussain KK, et al. Amperometric sensing of HIF1α expressed in cancer cells and the effect of hypoxic mimicking agents. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27132006/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001521

Uniprot Accession Number

Q16665

Species

Human

AA Sequence

MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN