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Recombinant human Glutaredoxin 1/GLRX1 protein

Description: Glutaredoxin (GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Mammalian Glutaredoxin is known to have two isoforms, GRX1 and GRX2. GRX1 is a cytosolic protein, whereas GRX2 is localized both in the mitochondria and nucleus. Glutaredoxin-1 may be involved in a various cellular events such as signal transduction, stress response, and metabolic regulation by regulating the redox status of cellular proteins. Recombinant human Glutaredoxin1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GLRX, GRX, GRX1, Thioltransferase-1, TTase-1, Glrx1, Glutaredoxin, Glutaredoxin1, Grx 1, MGC117407, Thioltransferase, Thioltransferase 1, Thioltransferase1, Ttase, TTase 1, TTase1.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

11.7 kDa (106aa) confirmed by MALDI-TOF

Additionnal Information

Glutaredoxin1, GLRX1, GLRX, GRX, GRX1, Thioltransferase-1, TTase-1, Glrx1, Glutaredoxin, Glutaredoxin1, Grx 1, MGC117407, Thioltransferase, Thioltransferase 1, Thioltransferase1, Ttase, TTase 1, TTase1., GRX0904-10 µg, GRX0904-20 µg, GRX0904-50 µg, GRX0904-100 µg, GRX0904-250 µg, GRX0904-500 µg, GRX0904-1 mg, GRX0904-10, GRX0904-20, GRX0904-50, GRX0904-100, GRX0904-250, GRX0904-500, GRX0904-1

References & Citations

Reynaert NL., et al. (2006) Proc Natl Acad Sci u S A. 103 (35) :13086-91.; ; Kanda M., et al. (2006) J Biol Chem. 281 (39) :28518-28.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_002055.1

Uniprot Accession Number

P35754

Species

Human

AA Sequence

MAQEFVNCKI QPGKVVVFIK PTCPYCRRAQ EILSQLPIKQ GLLEFVDITA TNHTNEIQDY LQQLTGARTV PRVFIGKDCI GGCSDLVSLQ QSGELLTRLK QIGALQ

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