Recombinant e.coli GrpE protein (V44I)

GrpE, co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.

Product Specifications

Product Name Alternative

HSP-70 cofactor, Heat shock protein B25.3, HSP24, GrpE, HSP 70 cofactor.

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.8 kDa (197aa) confirmed by MALDI-TOF

Additionnal Information

GrpE, HSP-70 cofactor, Heat shock protein B25.3, HSP24, GrpE, HSP 70 cofactor., GRP0701-10 µg, GRP0701-20 µg, GRP0701-50 µg, GRP0701-100 µg, GRP0701-250 µg, GRP0701-500 µg, GRP0701-1 mg, GRP0701-10, GRP0701-20, GRP0701-50, GRP0701-100, GRP0701-250, GRP0701-500, GRP0701-1

References & Citations

Lipinska B, King J, Ang D, Georgopoulos C (1988) . Nucleic Acids Res.16: 545-7562; ; Liberek K., Marszzalek J., Ang D, Georgopoulos C., Zylicz M. (1991) . Proc. Natl. Acad.Sci. u.S.A. 88: 2874-2878.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_417104.1

Uniprot Accession Number

P09372

Species

E.coli

AA Sequence

MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA