Recombinant E.coli GroEL protein

GroEL protein is the major heat shock protein of E. coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GroL, groEL, mopA, Protein Cpn60, 60 kDa chaperonin, groEL protein, chaperonin groel

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

57.3 kDa (548aa) confirmed by MALDI-TOF

Additionnal Information

GroEL, groL, groEL, mopA, Protein Cpn60, 60 kDa chaperonin, groEL protein, chaperonin groel, GRL3001-10 µg, GRL3001-20 µg, GRL3001-50 µg, GRL3001-100 µg, GRL3001-250 µg, GRL3001-500 µg, GRL3001-1 mg, GRL3001-10, GRL3001-20, GRL3001-50, GRL3001-100, GRL3001-250, GRL3001-500, GRL3001-1

References & Citations

Hemmingsen, S.M., et al (1988) Nature . 333 (6171) 330-334; ; Braig, K., et al (1994) Nature 371 (6498) 578-586; ; Chen, L and Singler, P.B. (1999) Cell 99 (7) 757-768;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5mM DTT, 10%glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_418567.1

Uniprot Accession Number

P0A6F5

Species

E.coli

AA Sequence

MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM