What sizes are available for Recombinant human Growth Hormone 1/GH1, 20kDa variant protein?

Recombinant human Growth Hormone 1/GH1, 20kDa variant protein is available in the following size(s): 500 µg.

Recombinant human Growth Hormone 1/GH1, 20kDa variant protein

Q: What is the catalog number for Recombinant human Growth Hormone 1/GH1, 20kDa variant protein?

The catalog number for Recombinant human Growth Hormone 1/GH1, 20kDa variant protein is GRH3002-500.

The 20KDa variant form of human growth hormone (20KDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22K, 191 amino acid) by the deletion of amino acid residues 32-46. The physiological role of 20K hGH remains to be determined partly but it regards the main function as stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant 20KDa human growth hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ≤1 ng/mL.

Product Name Alternative

Somatotropin isoform 2, 20kDa Growth Hormone, GH, GH1, GHN, GH-N, Growth hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤1 ng/mL.

Molecular Weight

20 kDa (177aa) confirmed by MALDI-TOF

Additionnal Information

GH, GH 1, GH1, GHN, GH-N, Growth Hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin, GRH3002-10 µg, GRH3002-20 µg, GRH3002-50 µg, GRH3002-100 µg, GRH3002-250 µg, GRH3002-500 µg, GRH3002-1 mg, GRH3002-010, GRH3002-020, GRH3002-050, GRH3002-100, GRH3002-250, GRH3002-500, GRH3002-01M

References & Citations

Frigeri, et al (1979) Biochem Biophys Res Commun. 14:778-82; ; Adelman, et al (1983) DNA. 2; 183-93; ; Tsushima, et al (2000) Endocr J. 47; S17-21; ; Horan, M., et al (2003) Hum. Mutat. 21 (4), 408-423;

Other References

Zhang H, et al. Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer. (Talanta. 2009) {https://pubmed.ncbi.nlm.nih.gov/19576433/}; ; Jung J, et al. Production and engineering of nanobody-based quenchbody sensors for detecting recombinant human growth hormone and its isoforms. (Drug Test Anal. 2023) {https://pubmed.ncbi.nlm.nih.gov/37667448/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_072053.1

Uniprot Accession Number

P01241

Species

Human

AA Sequence

MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF