Recombinant human Growth Hormone 1/GH1 protein
Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Its main function is the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant human growth hormone (rhGH) was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Specifications
Human Growth Hormon Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.2 ng/mL.
Product Name Alternative
GH, GH1, GHN, GH-N, Growth hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.2 ng/mL.
Molecular Weight
22.2 kDa (192aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 88mM mannitol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000506
Uniprot Accession Number
P01241
Species
Human
AA Sequence
Frequently Asked Questions
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