Recombinant human Geminin protein

Geminin is a 25 kDa protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions.

Product Specifications

Product Name Alternative

GMNN, Geminin, Geminin, DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, OTTHuMP00000039393, RP3 369A17.3.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

27.6 kDa (245aa) confirmed by MALDI-TOF

Additionnal Information

Geminin, GMNN, Geminin, Geminin, DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, OTTHuMP00000039393, RP3 369A17.3., GMN0601-10 µg, GMN0601-20 µg, GMN0601-50 µg, GMN0601-100 µg, GMN0601-250 µg, GMN0601-500 µg, GMN0601-1 mg, GMN0601-10, GMN0601-20, GMN0601-50, GMN0601-100, GMN0601-250, GMN0601-500, GMN0601-1

References & Citations

McGarry TJ. et al (1998) Cell. 93 (6) :1043-53.; ; Pitulescu M. et al (2005) Cell Mol Life Sci. 62 (13) :1425-33.; ; Luo L. et al (2004) Nature. 427 (6976) :749-53;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_056979.1

Uniprot Accession Number

O75496

Species

Human

AA Sequence

MNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD AKPCI