Recombinant human Glyoxalase I protein
Glyoxalase I, also known as GLO1, belongs to the glyoxalase I family. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
20.7 kDa (184aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ridderstrom M., et al. (1996) Biochem J 314 (Pt2) 463-7; ; Sakamoto H., et al. (2000) Blood 95 (10) : 3214-8;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_006699.2
Uniprot Accession Number
Q04760
Species
Human
AA Sequence
Frequently Asked Questions
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