Recombinant human Glyoxalase I protein

Glyoxalase I, also known as GLO1, belongs to the glyoxalase I family. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Lactoylglutathione lyase, Glx 1, GLO-1 Methylglyoxalase, Aldoketomutase, GLOD1, Glx I, GLYI, Glyoxalase I, Ketone aldehyde mutase, Lactoyl glutathione lyase, S D lactoylglutathione methylglyoxal lyase 0, Glyoxalase 1, GLO1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.7 kDa (184aa) confirmed by MALDI-TOF

Additionnal Information

Glyoxalase I, Lactoylglutathione lyase, Glx 1, GLO-1 Methylglyoxalase, Aldoketomutase, GLOD1, Glx I, GLYI, Glyoxalase I, Ketone aldehyde mutase, Lactoyl glutathione lyase, S D lactoylglutathione methylglyoxal lyase 0, Glyoxalase 1, GLO1, GLX0905-10 µg, GLX0905-20 µg, GLX0905-50 µg, GLX0905-100 µg, GLX0905-250 µg, GLX0905-500 µg, GLX0905-1 mg, GLX0905-10, GLX0905-20, GLX0905-50, GLX0905-100, GLX0905-250, GLX0905-500, GLX0905-1

References & Citations

Ridderstrom M., et al. (1996) Biochem J 314 (Pt2) 463-7; ; Sakamoto H., et al. (2000) Blood 95 (10) : 3214-8;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_006699.2

Uniprot Accession Number

Q04760

Species

Human

AA Sequence

MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM