Recombinant saccharomyces cerevisiae GLC8 protein
GLC8 is a regulatory subunit of protein phosphatase 1 (Glc7p) . This protein involved in glycogen metabolism and chromosome segregation, proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2. under normal conditions, Glc8p activates Glc7p, but when Glc8p is over produced, it may also inhibit Glc7p function. Glc8p is activated upon phosphoylation by Pho85p complexed with four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p) . Recombinant yeast GLC8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glc8p, GLC8
Expression System
E.coli
Antigen Species
Saccharomyces cerevisiae
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.7 kDa (265aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Tung HY., et al. (1995) Mol Cell Biol. 15 (11) :6064-74; ; Tan YS., et al. (2003) J Biol Chem. 278 (1) :147-53;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_014042
Uniprot Accession Number
P41818
Species
Saccharomyces cerevisiae
AA Sequence
Frequently Asked Questions
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