Recombinant saccharomyces cerevisiae GLC8 protein

GLC8 is a regulatory subunit of protein phosphatase 1 (Glc7p) . This protein involved in glycogen metabolism and chromosome segregation, proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2. under normal conditions, Glc8p activates Glc7p, but when Glc8p is over produced, it may also inhibit Glc7p function. Glc8p is activated upon phosphoylation by Pho85p complexed with four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p) . Recombinant yeast GLC8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glc8p, GLC8

Expression System

E.coli

Antigen Species

Saccharomyces cerevisiae

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.7 kDa (265aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

GLC8, Glc8p, GLC8, GLC0901-10 µg, GLC0901-20 µg, GLC0901-50 µg, GLC0901-100 µg, GLC0901-250 µg, GLC0901-500 µg, GLC0901-1 mg, GLC0901-10, GLC0901-20, GLC0901-50, GLC0901-100, GLC0901-250, GLC0901-500, GLC0901-1

References & Citations

Tung HY., et al. (1995) Mol Cell Biol. 15 (11) :6064-74; ; Tan YS., et al. (2003) J Biol Chem. 278 (1) :147-53;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_014042

Uniprot Accession Number

P41818

Species

Saccharomyces cerevisiae

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGGI LKNPLALSPE QLAQQDPETL EEFRRQVYEN TQKNAKLTSH KRNIPGLDNT KEEGEIIGTS STFLPKDTLS LKHEQDMLAK MTPEERVQWN QRNLAENEIT KKQFQDIHID EPKTPYQGAV DPHGEYYRVD DDEDEDNSDK KPCQVANDDI DDLSLGEPEF EIKENKQPDF ETNDENDEDS PEARHKKFEE MRKKHYDVRA IFNKKSREAL KDEDEDEDDS TTKEP