Recombinant human Gankyrin/PSMD10 protein

Gankyrin is an oncoprotein commonly overexpressed in most hepatocellular carcinomas. Gankyrin interacts with S6 ATPase of the 19S regulatory particle of the 26S proteasome. It is known to play a role in the regulation of the phosphorylation of the retinoblastoma protein by CDK4, and to enhance the ubiquitinylation of p53 by MDM2. Gankyrin consists of 7 ankyrin repeats and is structurally similar to I kappa Bs. Recombinant human Gankyrin was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.4 kDa (226aa) confirmed by MALDI-TOF

Additionnal Information

Gankyrin, GAN0901-10 µg, GAN0901-20 µg, GAN0901-50 µg, GAN0901-100 µg, GAN0901-250 µg, GAN0901-500 µg, GAN0901-1 mg, GAN0901-10, GAN0901-20, GAN0901-50, GAN0901-100, GAN0901-250, GAN0901-500, GAN0901-1

References & Citations

Umemura A., et al. (2008) Hepatology. 47 (2) :493-502.; ; Chen Y., et al. (2007) Cell Res. 17 (12) : 1020-9;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_002805

Uniprot Accession Number

O75832

Species

Human

AA Sequence

MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG