Recombinant human GADD45 gamma protein

GADD45G is a member of GADD45 family (GADD45alpha, beta, and gamma) that are nuclear proteins to interact with various proteins implicated in stress responses and cell-cycle-related proteins. The function of GADD45 proteins is involved in growth-regulatory mechanisms and apoptosis. GADD45G protein responds to environmental stresses by mediating activation of p38/JNK pathway via MTK1/MEKK4 kinase and its transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Recombinant GADD45G protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Growth arrest and DNA-damage-inducible gamma, CR6, DDIT2, Growth arrest and DNA-damage-inducible gamma, Growth arrest and DNA-damage-inducible gamma, Cytokine responsive protein CR6, GADD45G, Growth arrest and DNA damage inducible gamma, GRP17

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.8 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

GADD45 g, Growth arrest and DNA-damage-inducible gamma, CR6, DDIT2, Growth arrest and DNA-damage-inducible gamma, Growth arrest and DNA-damage-inducible gamma, Cytokine responsive protein CR6, GADD45G, Growth arrest and DNA damage inducible gamma, GRP17, GAD0902-10 µg, GAD0902-20 µg, GAD0902-50 µg, GAD0902-100 µg, GAD0902-250 µg, GAD0902-500 µg, GAD0902-1 mg, GAD0902-10, GAD0902-20, GAD0902-50, GAD0902-100, GAD0902-250, GAD0902-500, GAD0902-1

References & Citations

Takekawa M., et al. (1998) Cell 95 (4), 521-30; ; Zerbini LF., et al. (2004) PNAS 101 (37), 13618-23;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Cell Cycle

NCBI Accession Number

NP_006696.1

Uniprot Accession Number

O95257

Species

Human

AA Sequence

MTLEEVRGQD TVPESTARMQ GAGKALHELL LSAQRQGCLT AGVYESAKVL NVDPDNVTFC VLAAGEEDEG DIALQIHFTL IQAFCCENDI DIVRVGDVQR LAAIVGAGEE AGAPGDLHCI LISNPNEDAW KDPALEKLSL FCEESRSVND WVPSITLPE