Recombinant human Ferric uptake regulator/FuR protein

Fur (ferric uptake regulator) protein is a DNA-binding protein which regulates iron-responsive genes. Fur is a small, 17-kDa, global transcriptional repressor that in the presence of iron regulates functions as diverse as iron acquisition, oxidative stress, and virulence. In Escherichia coli, members of the Fur family regulate the expression of more than 100 genes that function in processes as varied as the biosynthesis and transport of siderophores, the expression of virulence factors, the alleviation of oxidative and NO-induced stress, and the inhibition of ferritin production through the expression of RyhB. Recombinant FuR was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-binding transcriptional dual regulator of siderophore biosynthesis and transport, Ferric uptake regulator, FuR, ECK0671, Ferric uptake regulation protein, JW0669.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.7 kDa (148aa) confirmed by MALDI-TOF

Additionnal Information

Ferric uptake regulator, FuR, FUR0801-10 µg, FUR0801-20 µg, FUR0801-50 µg, FUR0801-100 µg, FUR0801-250 µg, FUR0801-500 µg, FUR0801-1 mg, FUR0801-10, FUR0801-20, FUR0801-50, FUR0801-100, FUR0801-250, FUR0801-500, FUR0801-1

References & Citations

O'Sullivan DJ., et al. (1994) . FEMS Microbiol Lett. Apr 15; ; 117 (3) :327-32; ; Mills SA., et al. (2005) Biochemistry. Oct 18; ; 44 (41) :13553-9;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM CaCl2, 100 mM NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_415209

Uniprot Accession Number

P0A9A9

Species

Human

AA Sequence

MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI RLTNHSLYLY GHCAEGDCRE DEHAHEGK