Recombinant human Fumarase/FH protein

Specific activity is > 25 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Malate to Fumarate per minute at pH 7.5 at 37C.

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 25 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Malate to Fumarate per minute at pH 7.5 at 37C.

50.2 kDa (467aa) confirmed by MALDI-TOF

Lehtonen R., et al. (2003) J. Med. Genet. 40 (3) : e19.; ; Toro JR., et al. (2003) Am. J. Hum. Genet. 73 (1) : 95-106.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0)

Cell Cycle

NP_000134

P07954

Human