Recombinant human FABP5/E-FABP protein
FABP5 is a member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to specifically bind fatty acids (FAs) with high affinity for stearic and linoleic acids. FABP5 is expressed in endothelial cells of the microvasculature of the placenta, heart, skeletal muscle, small intestine, lung, and renal medulla. FABP4 and FABP5 are closely related and both are expressed in adipocytes. Absence of FABP5 resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance. Recombinant human FABP5 was expressed in E. coli and purified by conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
15.1 kDa (135aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hagens G., et al: (1999) Biochem.J ; 339 (Pt 2) : 419-27; ; Hohoff C., et al: (1999) Biochemistry ; 38 (38) : 12229-39
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol
Scientific Category
Serology
NCBI Accession Number
NP_001435
Uniprot Accession Number
Q01469
Species
Human
AA Sequence
MATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI YEKVE
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