Recombinant human EGF protein

Recombinant human epidermal growth factor (EGF) is a 6. 3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range is less or equal to 0.1 ng/mL.

Product Name Alternative

Epidermal growth factor, Pro-epidermal growth factor, urogastrone, EGF

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is less or equal to 0.1 ng/mL.

Molecular Weight

6.3 kDa (54aa) confirmed by MALDI-TOF

Additionnal Information

EGF, EGF0501-10 µg, EGF0501-20 µg, EGF0501-50 µg, EGF0501-100 µg, EGF0501-250 µg, EGF0501-500 µg, EGF0501-1 mg, EGF0501-10, EGF0501-20, EGF0501-50, EGF0501-100, EGF0501-250, EGF0501-500, EGF0501-1, epidermal growth factor (beta-urogastrone)

References & Citations

Riese., et al (1998) Bioessays. 20: 41-48.; ; Cohen S., (1983) Cancer. 15: 1787-1791. ; ; Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.;

Other References

Tazawa H, et al. Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells. (Exp Cell Res. 2013) {https://pubmed.ncbi.nlm.nih.gov/23948305/}; ; Park SY, et al. DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling. (Biochim Biophys Acta. 2015) {https://pubmed.ncbi.nlm.nih.gov/26239118/}; ; Okuda K, et al. Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis. (Nat Commun. 2023) {https://pubmed.ncbi.nlm.nih.gov/36739439/}