Recombinant human EBAG9 protein

Estrogen receptor binding site associated antigen 9, also known as EBAG9, is a type III transmembrane protein that is predominantly located in the Golgi. This protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers, such as advanced breast and prostate cancers. As the EBAG9 acts to inhibit the growth of receptor-binding cells and induced apoptosis of immune cells, cancer cells might evade immune surveillance. So immunodetection of EBAG9 expression can be a negative prognostic indicator. Recombinant human EBAG9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Estrogen receptor binding site associated antigen 9, EB9, PDAF, RCAS1, Estrogen receptor binding site associated antigen 9, EBAG9, Estrogen receptor binding site associated, antigen 9 BAG9, Cancer associated surface antigen, Cancer associated surface antigen RCAS1, EBAG 9, Estrogen receptor binding fragment associated gene 9 protein, PDAF, RCAS 1, RCAS1, Receptor binding cancer antigen expressed on SiSo cells.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.4 kDa (207aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

EBAG9, Estrogen receptor binding site associated antigen 9, EB9, PDAF, RCAS1, Estrogen receptor binding site associated antigen 9, EBAG9, Estrogen receptor binding site associated, antigen 9 BAG9, Cancer associated surface antigen, Cancer associated surface antigen RCAS1, EBAG 9, Estrogen receptor binding fragment associated gene 9 protein, PDAF, RCAS 1, RCAS1, Receptor binding cancer antigen expressed on SiSo cells., EBA0901-10 µg, EBA0901-20 µg, EBA0901-50 µg, EBA0901-100 µg, EBA0901-250 µg, EBA0901-500 µg, EBA0901-1 mg, EBA0901-10, EBA0901-20, EBA0901-50, EBA0901-100, EBA0901-250, EBA0901-500, EBA0901-1

References & Citations

Kumagai J., et al., (2009) Int J Cancer.124 (4) :816-26.; ; Yoshida S., et al., (2008) J Rheumatol. 35 (9) :1716-22.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 2mM EDTA, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_004206

Uniprot Accession Number

O00559

Species

Human

AA Sequence

RSGRGRKLS GDQITLPTTV DYSSVPKQTD VEEWTSWDED APTSVKIEGG NGNVATQQNS LEQLEPDYFK DMTPTIRKTQ KIVIKKREPL NFGIPDGSTG FSSRLAATQD LPFIHQSSEL GDLDTWQENT NAWEEEEDAA WQAEEVLRQQ KLADREKRAA EQQRKKMEKE AQRLMKKEQN KIGVKLS