Recombinant human CD26/DPP4 protein

DPP4, dipeptidyl peptidase-4 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) . Recombinant human DPP4 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 200 units/mg, One unit produces 1.0 umole of p-Nitroaniline from Gly-Pro- p-Nitroaniline per minute at pH 8.0 at 37C.

Product Name Alternative

Dipeptidyl peptidase 4, dipeptidyl peptidase 4 isoform 1, ADABP, ADCP2, CD26, DPPIV, TP103, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, DPP4, T-cell activation antigen CD26

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 200unit/mg, One unit produces 1.0 umole of p-Nitroaniline from Gly-Pro- p-Nitroaniline per minute at pH 8.0 at 37C.

Molecular Weight

85.4 kDa (737aa) confirmed by MALDI-TOF

Additionnal Information

TP103, T-cell activation antigen CD26, DPPIV, DPP4, Dipeptidylpeptidase IV (CD26 adenosine deaminase complexing protein 2), Dipeptidyl-peptidase 4, Dipeptidyl peptidase-4, Dipeptidyl peptidase 4, CD26, Adenosine deaminase complexing protein 2, ADCP2, ADABP, DPP0901-10 µg, DPP0901-20 µg, DPP0901-50 µg, DPP0901-100 µg, DPP0901-250 µg, DPP0901-500 µg, DPP0901-1 mg, DPP0901-010, DPP0901-020, DPP0901-050, DPP0901-100, DPP0901-250, DPP0901-500, DPP0901-01M

References & Citations

Pratley RE and Salsali A. (2007) Curr Med Res Opin. 23 (4) :919-31.; ; Rosenstock J. and Zinman B. (2007) Curr Opin Endocrino Diabetes Obes.60 (11) :1454-70.; ; Barnett A. (2006) . J.Clin. Pract. 60 (11) :1454-70.;

Other References

Hussein GM, et al. Mate tea (Ilex paraguariensis) promotes satiety and body weight lowering in mice: involvement of glucagon-like peptide-1. (Biol Pharm Bull. 2011) {https://pubmed.ncbi.nlm.nih.gov/22130241/}; ; Sato Y, et al. The role of dipeptidyl peptidase 4 (DPP4) in the preservation of renal function: DPP4 involvement in hemoglobin expression. (J Endocrinol. 2014) {https://pubmed.ncbi.nlm.nih.gov/25122001/}; ; Roppongi S, et al. Crystal structures of a bacterial dipeptidyl peptidase IV reveal a novel substrate recognition mechanism distinct from that of mammalian orthologues. (Sci Rep. 2018) {https://pubmed.ncbi.nlm.nih.gov/29426867/}; ; Guan C, et al. Strategic Preparations of DPP-IV Inhibitory Peptides from Val-Pro-Xaa and Ile-Pro-Xaa Peptide Mixtures. (Int J Pept Res Ther. 2020) {https://pubmed.ncbi.nlm.nih.gov/25122001/}; ; Guan C, et al. Exploration of DPP-IV Inhibitory Peptide Design Rules Assisted by the Deep Learning Pipeline That Identifies the Restriction Enzyme Cutting Site. (ACS Omega. 2023) {https://pubmed.ncbi.nlm.nih.gov/37901493/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1mM EDTA, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001926.2

Uniprot Accession Number

P27487

Species

Human

N Terminal Sequence

Ala

AA Sequence

SRKTYTL TDYLKNTYRL KLYSLRWISD HEYLYKQENN ILVFNAEYGN SSVFLENSTF DEFGHSINDY SISPDGQFIL LEYNYVKQWR HSYTASYDIY DLNKRQLITE ERIPNNTQWV TWSPVGHKLA YVWNNDIYVK IEPNLPSYRI TWTGKEDIIY NGITDWVYEE EVFSAYSALW WSPNGTFLAY AQFNDTEVPL IEYSFYSDES LQYPKTVRVP YPKAGAVNPT VKFFVVNTDS LSSVTNATSI QITAPASMLI GDHYLCDVTW ATQERISLQW LRRIQNYSVM DICDYDESSG RWNCLVARQH IEMSTTGWVG RFRPSEPHFT LDGNSFYKII SNEEGYRHIC YFQIDKKDCT FITKGTWEVI GIEALTSDYL YYISNEYKGM PGGRNLYKIQ LSDYTKVTCL SCELNPERCQ YYSVSFSKEA KYYQLRCSGP GLPLYTLHSS VNDKGLRVLE DNSALDKMLQ NVQMPSKKLD FIILNETKFW YQMILPPHFD KSKKYPLLLD VYAGPCSQKA DTVFRLNWAT YLASTENIIV ASFDGRGSGY QGDKIMHAIN RRLGTFEVED QIEAARQFSK MGFVDNKRIA IWGWSYGGYV TSMVLGSGSG VFKCGIAVAP VSRWEYYDSV YTERYMGLPT PEDNLDHYRN STVMSRAENF KQVEYLLIHG TADDNVHFQQ SAQISKALVD VGVDFQAMWY TDEDHGIASS TAHQHIYTHM SHFIKQCFSL P