Recombinant E.coli Dnak (385-638aa) protein

DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. The protein coding region of the substrate binding domain of DNAK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus

Product Specifications

Product Name Alternative

Dnak (C-term, 385-638), Substrate binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp 70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27.7 kDa (255aa) confirmed by MALDI-TOF

Additionnal Information

Dnak, Dnak (C-term, 385-638), Substrate binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp 70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein, DNK3003-10 µg, DNK3003-20 µg, DNK3003-50 µg, DNK3003-100 µg, DNK3003-250 µg, DNK3003-500 µg, DNK3003-1 mg, DNK3003-10, DNK3003-20, DNK3003-50, DNK3003-100, DNK3003-250, DNK3003-500, DNK3003-1

References & Citations

Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852; ; Zhu et al., (1996) Science 272, 1606-1614.; ; Naoki tanaka., et al (2002) PNAS 26 (99) 15398-15403;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5mM DTT, 10%glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_414555

Uniprot Accession Number

P0A6Y8

Species

E.coli

AA Sequence

MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HSTRKQVEEA GDKLPADDKT AIESALTALE TALKGEDKAA IEAKMQELAQ VSQKLMEIAQ QQHAQQQTAG ADASANNAKD DDVVDAEFEE VKDKK