Recombinant E.coli Dnak (1-384aa) protein

DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain (residues385-638) . The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques.

Product Specifications

Product Name Alternative

DnaK, Dnak (N-term, 1-384), ATPase binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp70, Co chaperone with DnaJ

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

41.6 kDa (384aa)

Additionnal Information

Dnak, dnaK, Dnak (N-term, 1-384), ATPase binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp70, Co chaperone with DnaJ, DNK3002-10 µg, DNK3002-20 µg, DNK3002-50 µg, DNK3002-100 µg, DNK3002-250 µg, DNK3002-500 µg, DNK3002-1 mg, DNK3002-10, DNK3002-20, DNK3002-50, DNK3002-100, DNK3002-250, DNK3002-500, DNK3002-1

References & Citations

Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852; ; Zhu et al., (1996) Science. 272, 1606-1614.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5mM DTT, 10%glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_414555

Uniprot Accession Number

P0A6Y8

Species

E.coli

AA Sequence

MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG