Recombinant E.coli Dnak (385-546aa) protein

DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus

Product Specifications

Product Name Alternative

Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.7 kDa (163aa)

Additionnal Information

Dnak, Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein, DNK2001-10 µg, DNK2001-20 µg, DNK2001-50 µg, DNK2001-100 µg, DNK2001-250 µg, DNK2001-500 µg, DNK2001-1 mg, DNK2001-10, DNK2001-20, DNK2001-50, DNK2001-100, DNK2001-250, DNK2001-500, DNK2001-1

References & Citations

Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852; ; Zhu et al., (1996) Science 272, 1606-1614.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_414555

Uniprot Accession Number

P0A6Y8

Species

E.coli

AA Sequence

MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST