Recombinant E.coli DnaJ protein

DnaJ, Heat shock protein, functions in association with DnaK (Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70.. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues. DnaJ (amino acids 1-376) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.

Product Specifications

Product Name Alternative

DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4, DnaJ (1-376aa), Heat shock 40 kDa protein 4, DnaJ protein homolog 2, HDJ-2, HSJ-2, HSDJ, DnaJ homolog subfamily A member 1

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

41.1 kDa (376aa) confirmed by MALDI-TOF

Additionnal Information

DnaJ, DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4, DnaJ (1-376aa), Heat shock 40 kDa protein 4, DnaJ protein homolog 2, HDJ-2, HSJ-2, HSDJ, DnaJ homolog subfamily A member 1, DNJ3001-10 µg, DNJ3001-20 µg, DNJ3001-50 µg, DNJ3001-100 µg, DNJ3001-250 µg, DNJ3001-500 µg, DNJ3001-1 mg, DNJ3001-10, DNJ3001-20, DNJ3001-50, DNJ3001-100, DNJ3001-250, DNJ3001-500, DNJ3001-1

References & Citations

Bardwell, J.C., et al (1986) J.Biol.Chem.261 (4) ; ;1782-1785; ; Ohki M., et al (1986) J.Biol.Chem.261 (4) ; ;1778-1781; ; Zylicz M., et.al. (1989) EMBO J 8 (5) 1601-8 ; ; Landry SJ., et al. (2003) Biochemistry 42 (17) 4926-36;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 25mM Tris-HCl buffer (pH 8.8) containing 5mM DTT, 100 mM NaCl, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_414556

Uniprot Accession Number

P08622

Species

E.coli

AA Sequence

MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR