Recombinant human Desert Hedgehog/Dhh N-terminus protein

DHH belongs to the hedgehog family. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N- terminal domain is the active species in both local and long-range signaling, whereas the C-terminal domain has no signaling activity. This protein is produced by Sertoli cells and may be involved in both male gonadal differentiation and perineurial development. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. Recombinant DHH protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Desert hedgehog preproprotein, Drosophila, HHG-3, MGC35145, Desert hedgehog protein N-product, DHH, desert hedgehog preproprotein Desert hedgehog, Desert hedgehog (Drosophila) homolog, Desert hedgehog homolog (Drosophila), Desert hedgehog protein precursor.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

22 kDa (197aa) confirmed by MALDI-TOF

Additionnal Information

Desert Hedgehog, Dhh N-terminus, desert hedgehog preproprotein, Drosophila, HHG-3, MGC35145, Desert hedgehog protein N-product, DHH, desert hedgehog preproprotein Desert hedgehog, Desert hedgehog (Drosophila) homolog, Desert hedgehog homolog (Drosophila), Desert hedgehog protein precursor., DHH0901-10 µg, DHH0901-20 µg, DHH0901-50 µg, DHH0901-100 µg, DHH0901-250 µg, DHH0901-500 µg, DHH0901-1 mg, DHH0901-10, DHH0901-20, DHH0901-50, DHH0901-100, DHH0901-250, DHH0901-500, DHH0901-1

References & Citations

Chen YJ, et al. (2007) . Cell Cycle. 6 (15) :1826-30; ; Van den Brink GR., et al. (2007) . Physiol Rev. 87 (4) :1343-75;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 5.5) containing 0.5mM DTT, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_066382

Uniprot Accession Number

O43323

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MCGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG