Recombinant human Desert Hedgehog/Dhh N-terminus protein
DHH belongs to the hedgehog family. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N- terminal domain is the active species in both local and long-range signaling, whereas the C-terminal domain has no signaling activity. This protein is produced by Sertoli cells and may be involved in both male gonadal differentiation and perineurial development. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. Recombinant DHH protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
22 kDa (197aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chen YJ, et al. (2007) . Cell Cycle. 6 (15) :1826-30; ; Van den Brink GR., et al. (2007) . Physiol Rev. 87 (4) :1343-75;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM MES buffer (pH 5.5) containing 0.5mM DTT, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_066382
Uniprot Accession Number
O43323
Species
Human
AA Sequence
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