Recombinant human Cyclophilin 40/PPID protein

Cyclophilin D (also known as peptidylpropyl isomerase D, PPID) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression suppresses the apoptosis in cancer cells. Recombinant human cyclophilin D was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase D, Peptidylprolyl isomerase D, 40 kDa peptidyl-prolyl cis-trans isomerase, PPIase D, Cyclophilin-related protein, Rotamase D, CYP-40, CypD, Cyclophilin D

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

42.9 kDa (390aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin40, PPID, Peptidylprolyl isomerase D, PPID, CYPD, CYP-40, Cyclophilin D, 40 kDa peptidyl prolyl cis trans isomerase, 40 kDa peptidyl prolyl cis trans isomerase D, Cyclophilin related protein, Cyclophilin40, CyclophilinD, CYP 40, CYP40, MGC33096.Peptidylprolyl isomerase D, PPIase, PPID, Rotamase., CYP0801-10 µg, CYP0801-20 µg, CYP0801-50 µg, CYP0801-100 µg, CYP0801-250 µg, CYP0801-500 µg, CYP0801-1 mg, CYP0801-10, CYP0801-20, CYP0801-50, CYP0801-100, CYP0801-250, CYP0801-500, CYP0801-1

References & Citations

Machida K., et al. (2006) J. Biol. Chem. 281 (20) :14314-20. ; ; Yokoi H., et al. (1996) Genomics 35 (3) :448-55. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_005029

Uniprot Accession Number

Q08752

Species

Human

AA Sequence

MSHPSPQAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD SHPDFPEDAD IDLKDVDKIL LITEDLKNIG NTFFKSQNWE MAIKKYAEVL RYVDSSKAVI ETADRAKLQP IALSCVLNIG ACKLKMSNWQ GAIDSCLEAL ELDPSNTKAL YRRAQGWQGL KEYDQALADL KKAQGIAPED KAIQAELLKV KQKIKAQKDK EKAVYAKMFA