Recombinant human Cyclophilin A/PPIA protein

Cyclophilin A (also known as Peptidylprolyl isomerase A, PPIA) encodes a member of the peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. Cyclophilin A is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. Recombinant human PPIA, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 650 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, Cyclophilin A, Cyclosporin A binding protein, Peptidyl prolyl cis trans isomerase A, PPIA, PPIase A, Rotamase A, T cell cyclophilin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 650 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

20kDa (185aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin A, PPIA, Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, Cyclophilin A, Cyclosporin A binding protein, Peptidyl prolyl cis trans isomerase A, PPIA, PPIA protein, PPIase A, Rotamase A, RotamaseA, T cell cyclophilin., CYP0702-10 µg, CYP0702-20 µg, CYP0702-50 µg, CYP0702-100 µg, CYP0702-250 µg, CYP0702-500 µg, CYP0702-1 mg, CYP0702-10, CYP0702-20, CYP0702-50, CYP0702-100, CYP0702-250, CYP0702-500, CYP0702-1

References & Citations

Holzman, T.F., et al, (1991) . J. Biol. Chem. 266 (4) : 2474-2479; ; Berthoux L , et al, (2005) . Proc Natl Acad Sci u S A. 102 (41) :14849-53;

Other References

Gallay PA, et al. The Novel Cyclophilin Inhibitor CPI-431-32 Concurrently Blocks HCV and HIV-1 Infections via a Similar Mechanism of Action. (PLoS One. 2015) {https://www.ncbi.nlm.nih.gov/pubmed/26263487}; ; Gallay PA, et al. Characterization of the Anti-HCV Activities of the New Cyclophilin Inhibitor STG-175. (PLoS One. 2016) {https://www.ncbi.nlm.nih.gov/pubmed/27104614}; ; Kim K, et al. Recombinant human Cyclophilin A stimulates hair follicle cells via Wnt/β-catenin signaling pathway. (Biotechnol Lett. 2019) {https://pubmed.ncbi.nlm.nih.gov/31655924/}; ; Tsuda T, et al. Rho-associated protein kinase and cyclophilin a are involved in inorganic phosphate-induced calcification signaling in vascular smooth muscle cells. (J Pharmacol Sci. 2020) {https://pubmed.ncbi.nlm.nih.gov/31882204/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20 mM NaCl, 0.5mM DTT, 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_066953.1

Uniprot Accession Number

P62937

Species

Human

AA Sequence

MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE