Recombinant human Cyclophilin B/PPIB protein

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Specifications

Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

21.2 kDa (192aa) confirmed by MALDI-TOF

Additionnal Information

26- 216aa, 26-216aa, AA408962, AA553318, AI844835, Cphn 2, Cphn2, Cphn-2, Cyclophilin B, Cyclophilin-B, CyclophilinB, CyP 20b, CyP-20b, CyP20b, CYP S1, CYP-S1, CYPS1, CYPB, CYP-S1: Cyclophilin-B, MGC14109, MGC2224, Peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase, PPIB, Rotamase, S cyclophilin, SCYLP, CYP0701-10 µg, CYP0701-20 µg, CYP0701-50 µg, CYP0701-100 µg, CYP0701-250 µg, CYP0701-500 µg, CYP0701-1 mg, CYP0701-010, CYP0701-020, CYP0701-050, CYP0701-100, CYP0701-250, CYP0701-500, CYP0701-01M

References & Citations

Watashi K, et al. (2005) Mol Cell. 19 (1) :111-22. ; ; Obata Y, et al. (2005) J Biol Chem. 280 (18) :18355-60; ; Denys A , et al. (1997) Immunology. 91 (4) :609-17.;

Other References

Kim K, et al. Release of overexpressed CypB activates ERK signaling through CD147 binding for hepatoma cell resistance to oxidative stress. (Apoptosis. 2012) {https://pubmed.ncbi.nlm.nih.gov/22555451/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20 mM NaCl, 0.5mM DTT, 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_000933

Uniprot Accession Number

P23284

Species

Human

AA Sequence

MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE