Recombinant human G-CSF protein

Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human G-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3 ng/mL.

Product Name Alternative

Granulocyte-colony stimulating factor, CSF3, GCSF, G-CSF, G-CSF, Pluripoietin, Filgrastim, Lenograstim, Granulocyte colony-stimulating factor, Granulocyte-colony stimulating factor Colony stimulating factor 3 (granulocyte), CSF 3, CSF beta, Csfg, GCSA, Granulocyte colony stimulating factor, Lenograstim, Macrophage granulocyte inducer 2, MGC45931, MGI 2

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3 ng/mL.

Molecular Weight

18.8 kDa (175aa) confirmed by MALDI-TOF

Additionnal Information

G-CSF, CSF4001-10 µg, CSF4001-20 µg, CSF4001-50 µg, CSF4001-100 µg, CSF4001-250 µg, CSF4001-500 µg, CSF4001-1 mg, CSF4001-10, CSF4001-20, CSF4001-50, CSF4001-100, CSF4001-250, CSF4001-500, CSF4001-1

References & Citations

Nomura H, et al. (1986) EMBO J , 5 (5) : 871-6; ; Nagata, S., et al. (1986) Nature, 319 : 415-418; ; Demetri, G. D., et al. (1991) Blood, 78: 2791-2808 ; ; Hill, C. P., et al (1993) Proc. Natl. Acad. Sci. uSA, 90:5167-5171;

Other References

Lee EN, et al. Stabilizing peptide fusion for solving the stability and solubility problems of therapeutic proteins. Pharm Res. 2005 Oct;22 (10) :1735-46.{https://pubmed.ncbi.nlm.nih.gov/16180132/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_757373.1

Uniprot Accession Number

P09919

Species

Human

AA Sequence

MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP