Recombinant mouse Crystallin alpha B/CRYAB protein

Alpha crystallins are composed of two gene products, alpha-A and alpha-B for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) . They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. CRYAB is expressed widely in many tissues and organs. Recombinant CRYAB was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Crya2, Crya-2, Cryab, Crystallin alpha B, HspB5

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

20kDa (175aa) confirmed by MALDI-TOF

Additionnal Information

Crystallin a B, Crya2, Crya-2, Cryab, Crystallin alpha B, CRB0801-10 µg, CRB0801-20 µg, CRB0801-50 µg, CRB0801-100 µg, CRB0801-250 µg, CRB0801-500 µg, CRB0801-1 mg, CRB0801-10, CRB0801-20, CRB0801-50, CRB0801-100, CRB0801-250, CRB0801-500, CRB0801-1

References & Citations

Hasan, A., et al. (2002) Biochemistry 41 (52) 15876-15882; ; Kamradt, M.C., et al. (2002) J. Biol.Chem. 277 (41) 38731-38736 ; ; Reddy, G.B., et al. (2002) FEBS lett. 522 (1-3) 59-64 ; ; Ito, H., et al. (2002) J.Biochem.131 (4) 593-603;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_034094.1

Uniprot Accession Number

P23927

Species

Mouse

AA Sequence

MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK