Recombinant human Crystallin alpha B/CRYAB protein

Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) . They act as molecular chaperones and hold them in in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-B is expressed widely in many tissues and organs and occurs in many neurological diseases.

Product Specifications

Product Name Alternative

CRYAB, CRYA2, CRYAB (Crystallin alpha B), Rosenthal fiber component, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Alpha crystallin B chain, AACRYA, Alpha (B) crystallin, Crystallin alpha polypeptide 2, CTPP 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, NY REN 27 antigen, Renal carcinoma antigen NY REN 27

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.1 kDa (175aa)

Additionnal Information

AACRYA, Alpha crystallin B chain, Alpha (B) crystallin, Alpha-crystallin B chain, Crya2, Crya-2, Cryab, CRYAB (Crystallin alpha B), Crystallin alpha B, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, NY REN 27 antigen, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, CRA3002-10 µg, CRA3002-20 µg, CRA3002-50 µg, CRA3002-100 µg, CRA3002-250 µg, CRA3002-500 µg, CRA3002-1 mg, CRA3002-010, CRA3002-020, CRA3002-050, CRA3002-100, CRA3002-250, CRA3002-500, CRA3002-01M

References & Citations

Hasan, A., et al. (2002) Biochemistry 41 (52) 15876-15882; ; Kamradt, M.C.., et al (2002) J. Biol.Chem. 277 (41) 38731-38736 ; ; Reddy, G.B., et al (2002) FEBS lett. 522 (1-3) 59-64 ; ; Ito, H., et al (2002) J.Biochem.131 (4) 593-603;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 1mM EDTA

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_001876.1

Uniprot Accession Number

P02511

Species

Human

AA Sequence

MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK