Recombinant human Crystallin alpha A/CRYAA protein

Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) . They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. The expression of alpha-A is preferentially restricted to the lens cell.

Product Specifications

Product Name Alternative

Acry-1, Alpha crystallin A chain, CRYA1, CRYAA, Crystallin alpha 1, Crystallin alpha A, Heat shock protein beta 4, HSPB 4, HspB4, Zonular Central Nuclear Cataract

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.9 kDa (173aa)

Additionnal Information

A A Crystallin, Acry-1, Alpha crystallin A chain, Alpha-crystallin A chain, CRYA 1, CRYA1, CRYAA, Crystallin Alpha 1, Crystallin alpha A, Heat shock protein beta 4, Heat shock protein beta-4, HSPB 4, HspB4, Zonular Central Nuclear Cataract, CRA3001-10 µg, CRA3001-20 µg, CRA3001-50 µg, CRA3001-100 µg, CRA3001-250 µg, CRA3001-500 µg, CRA3001-1 mg, CRA3001-010, CRA3001-020, CRA3001-050, CRA3001-100, CRA3001-250, CRA3001-500, CRA3001-01M

References & Citations

Hasan, A., et al. (2002) Biochemistry 41 (52) 15876-15882; ; Bera, S., et al (2002) Biochemistry 41 (41) 12421-12426 ; ; Reddy, G.B., et al (2002) FEBS lett. 522 (1-3) 59-64 ; ; Andley, u.P., et al (2002) J.Biol.Chem.277 (12) 10178-10186;

Other References

Fan Q, et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. (Mol Vis. 2014) {https://pubmed.ncbi.nlm.nih.gov/24453475/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 1mM EDTA

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_000385.1

Uniprot Accession Number

P02489

Species

Human

AA Sequence

MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRL PSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS