Recombinant human Complexin-1 protein
Complexin1 belongs to a family of SNARE complex binding proteins that were suggested to be either facilitators or inhibitors of vesicle exocytosis. Complexin 1 exhibits reduced Ca2+-triggered fast neurotransmitter release at hippocampal glutamatergic synapses, indicating that this protein is a positive regulator of transmitter release. In contrast, it inhibits SNARE-mediated liposome and cell fusions in vitro, which lead to the hypothesis that it acts as fusion clamps of synaptic exocytosis. Recombinant Complexin 1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
CPXL1, CPX1, Synaphin-2, Complexin 1, Complexin I, CPX1, Synaphin 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.1 kDa (154aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Salimi K, et al. (2008) Synapse, 273-82; ; Giraudo CG, et al. (2006) Science, 676-80; ; Tang J, et al. (2006) Cell, 1175-87;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_006642
Uniprot Accession Number
O14810
Species
Human
AA Sequence
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