Recombinant human Complexin-1 protein

Complexin1 belongs to a family of SNARE complex binding proteins that were suggested to be either facilitators or inhibitors of vesicle exocytosis. Complexin 1 exhibits reduced Ca2+-triggered fast neurotransmitter release at hippocampal glutamatergic synapses, indicating that this protein is a positive regulator of transmitter release. In contrast, it inhibits SNARE-mediated liposome and cell fusions in vitro, which lead to the hypothesis that it acts as fusion clamps of synaptic exocytosis. Recombinant Complexin 1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CPXL1, CPX1, Synaphin-2, Complexin 1, Complexin I, CPX1, Synaphin 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

17.1 kDa (154aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Complexin1, CPXL1, CPX1, Synaphin-2, Complexin 1, Complexin I, CPX1, Synaphin 2, CPX0901-10 µg, CPX0901-20 µg, CPX0901-50 µg, CPX0901-100 µg, CPX0901-250 µg, CPX0901-500 µg, CPX0901-1 mg, CPX0901-10, CPX0901-20, CPX0901-50, CPX0901-100, CPX0901-250, CPX0901-500, CPX0901-1

References & Citations

Salimi K, et al. (2008) Synapse, 273-82; ; Giraudo CG, et al. (2006) Science, 676-80; ; Tang J, et al. (2006) Cell, 1175-87;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_006642

Uniprot Accession Number

O14810

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEVE EEDESILDTV IKYLPGPLQD MLKK