What is the catalog number for Recombinant human COMT protein?

The catalog number for Recombinant human COMT protein is COM0905-100.

What sizes are available for Recombinant human COMT protein?

Recombinant human COMT protein is available in the following size(s): 100 µg.

Is Recombinant human COMT protein in stock?

Recombinant human COMT protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human COMT protein

Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Catechol-O-methyltransferase, COMT

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.4 kDa (221aa) confirmed by MALDI-TOF

Additionnal Information

COMT, Catechol-O-methyltransferase, Catechol-O-methyltransferase, Catechol-O-methyltransferase, COMT, COM0905-10 µg, COM0905-20 µg, COM0905-50 µg, COM0905-100 µg, COM0905-250 µg, COM0905-500 µg, COM0905-1 mg, COM0905-10, COM0905-20, COM0905-50, COM0905-100, COM0905-250, COM0905-500, COM0905-1

References & Citations

Cotton NJ., et al. (2004) J Biol Chem. 279 (22) :23710-8. ; ; Dawling S., et al. (2001) Cancer Res. 61 (18) :6716-22.;

Other References

Ishida Y, et al. Yokukansan, a Traditional Japanese Medicine, Enhances the L-DOPA-Induced Rotational Response in 6-Hydroxydopamine-Lesioned Rats: Possible Inhibition of COMT. (Biol Pharm Bull. 2016) {https://pubmed.ncbi.nlm.nih.gov/29707715/}; ; Ikeda M, et al. Inhibitory Effect of Bovine Lactoferrin on Catechol-O-Methyltransferase. (Molecules. 2017) {https://pubmed.ncbi.nlm.nih.gov/28825621/}; ; Ikeda M, et al. Effects of bovine lactoferrin on l-DOPA absorption and metabolism in mice. (Food Funct. 2018) {https://pubmed.ncbi.nlm.nih.gov/26725433/}; ; Nagai M, et al. Comparative examination of levodopa pharmacokinetics during simultaneous administration with lactoferrin in healthy subjects and the relationship between lipids and COMT inhibitory activity in vitro. (Nutr Neurosci. 2020) {https://pubmed.ncbi.nlm.nih.gov/32441567/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM MgCl2, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000745.1

Uniprot Accession Number

P21964

Species

Human

AA Sequence

MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P