Recombinant human Cofilin 1/CFL1 protein
Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. Recombinant human Cofilin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Specifications
Product Name Alternative
18 kDa phosphoprotein (p18), CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
20kDa (186aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Actin & Myosin Related
NCBI Accession Number
NP_005498
Uniprot Accession Number
P23528
Species
Human
AA Sequence
Frequently Asked Questions
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