Recombinant human Cofilin 1/CFL1 protein

Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. Recombinant human Cofilin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

18 kDa phosphoprotein (p18), CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

20kDa (186aa) confirmed by MALDI-TOF

Additionnal Information

18 kDa phosphoprotein, 18 kDa phosphoprotein (p18), 18kDa phosphoprotein (p18), CFL, CFL-1, CFL 1, CFL1, Cofilin 1, Cofilin1, Cofilin-1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18, COF0701-10 µg, COF0701-20 µg, COF0701-50 µg, COF0701-100 µg, COF0701-250 µg, COF0701-500 µg, COF0701-1 mg, COF0701-010, COF0701-020, COF0701-050, COF0701-100, COF0701-250, COF0701-500, COF0701-01M

References & Citations

Mannherz H.G., et al (2007) . J. Mol. Biol. 366 (3) 745-755. ; ; Olsen J.V., et al (2006) . Cell. 127 (3) 635-648; ; Yang N., et al (1998) Nature. 393 (6687) 809-812;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Actin & Myosin Related

NCBI Accession Number

NP_005498

Uniprot Accession Number

P23528

Species

Human

AA Sequence

MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL