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Recombinant human CKS2 protein

CKS2, also known as CDC28 Protein kinase 2 associates with the catalytic subunit of cyclin-dependent kinases and has therefore been assumed to play a direct role in cell cycle regulation. The function of CKS2 in somatic mammalian cells is not well understood although it is required for the first metaphase/anaphase transition during the meiosis. Emerging evidence shows that elevated expression of CKS2 protects the cells from apoptosis. Recombinant CKS2 fused with T7-tag was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cyclin-dependent kinases regulatory subunit 2, CKSHS2, CKS2, CKS1 (S. cerevisiae Cdc28/Cdc2 kinase subunit) homolog 2, CDC28 protein kinase regulatory subunit 2, CDC28 Protein kinase 2

Expression System

E.coli

Antigen Species

Human

Tag

T7-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

11.3 kDa (94aa) confirmed by MALDI-TOF

Additionnal Information

Cyclin-dependent kinases regulatory subunit 2, Cyclin dependent kinases regulatory subunit 2, CKSHS2, CKS2, CKS1 (S. cerevisiae Cdc28/Cdc2 kinase subunit) homolog 2, CKS 2, CDC28 protein kinase regulatory subunit 2, CDC28 Protein kinase 2, , CKS0801-10 µg, CKS0801-20 µg, CKS0801-50 µg, CKS0801-100 µg, CKS0801-250 µg, CKS0801-500 µg, CKS0801-1 mg, CKS0801-010, CKS0801-020, CKS0801-050, CKS0801-100, CKS0801-250, CKS0801-500, CKS0801-01M

References & Citations

Martinsson-Ahlzen HS., et al: (2008) Mol Cell Biol. 28 (18) :5698-709.; ; Lan Y., et al: (2008) Int J Cancer. 123 (3) :543-51.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001818

Uniprot Accession Number

P33552

Species

Human

AA Sequence

MASMTGGQQM GRGSHMAHKQ IYYSDKYFDE HYEYRHVMLP RELSKQVPKT HLMSEEEWRR LGVQQSLGWV HYMIHEPEPH ILLFRRPLPK DQQK

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