Recombinant human BMP-4 (monomer) protein
Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family which belongs to the TGF-beta superfamily. This protein is a vital regulatory molecule that functions throughout bone and cartilage development, specifically tooth development, limb formation, bone induction, and fracture repair. In human embryonic development, BMP- 4 is required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. And it is overexpressed in patients with fibrodysplasia ossificans progressiva. Recombinant BMP-4 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
ZYME, DVR4, Bone morphogenetic protein 4, Bone morphogenetic protein 2B, BMP4, BMP2B1, BMP2B
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
13.2 kDa (117aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Moon BS., et al. (2009) Exp Mol Med. 41 (2) :116-25.; ; Li BC., et al. (2009) J Trauma. 66 (2) :450-6;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001193.2
Uniprot Accession Number
P12644
Species
Human
AA Sequence
MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
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