Recombinant human GRP78/HSPA5 protein
Binding immunoglobulin protein (BiP) belongs to the family of ~70 kDa heat shock proteins (HSP 70) . It is a stress response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding as well as for the maintenance of cell homeostasis and the prevention of apoptosis. Recombinant human BIP protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
71 kDa (640aa)
Additionnal Information
References & Citations
Lee LC., et al. (2009) Clin Chim Acta. 400 (1-2) :56-62.; ; Racek T., et al. (2008) J Biol Chem. 283 (49) :34305-14.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_005338.1
Uniprot Accession Number
P11021
Species
Human
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