Recombinant human BIGH3/TGFBI protein

BIGH3, also known as TGFBI and Beta ig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF) -beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain) . Recombinant human BIGH3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP, BIGH3, CSD3, LCD1, CSD1, CSD2, Transforming growth factor, Beta-induced, 68kD, CDB1, CDGG1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.9 kDa (182aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

B IG-H3, BIGH3, Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, TGFBI, Kerato-epithelin, RGD-CAP, Transforming growth factor-beta-induced protein ig-h3 RGD containing collagen associated protein, Beta ig h3, AI747162, CSD3, Kerato epithelin, LCD1, MGC150270, RGD CAP, TGFBI transforming growth factor, beta induced, 68kDa, AI181842, Beta ig, Big h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, EBMD, Transforming growth factor beta induced protein ig h3., BIG0905-10 µg, BIG0905-20 µg, BIG0905-50 µg, BIG0905-100 µg, BIG0905-250 µg, BIG0905-500 µg, BIG0905-1 mg, BIG0905-10, BIG0905-20, BIG0905-50, BIG0905-100, BIG0905-250, BIG0905-500, BIG0905-1

References & Citations

Billings PC., et al. (2002) J Biol Chem. 277, 28003-9. ; ; Munier FL., et al. (2002) Invest Ophthalmol Vis Sci. 43, 949-54. ; ; Kim JE., et al. (2000) J Biol Chem. 275, 30907-15.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA 0.1mM PMSF, 20% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_000349

Uniprot Accession Number

Q15582

Species

Human

AA Sequence

MGTVMDVLKG DNRFSMLVAA IQSAGLTETL NREGVYTVFA PTNEAFRALP PRERSRLLGD AKELANILKY HIGDEILVSG GIGALVRLKS LQGDKLEVSL KNNVVSVNKE PVAEPDIMAT NGVVHVITNV LQPPANRPQE RGDELADSAL EIFKQASAFS RASQRSVRLA PVYQKLLERM KH