Recombinant human BHMT protein

Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis. Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Betaine-homocysteine S-methyltransferase 1, BHMT1, Betaine homocysteine methyltransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

49.2 kDa (443aa) confirmed by MALDI-TOF

Additionnal Information

Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine-homocysteine methyltransferase, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine-homocysteine S-methyltransferase 1, BHMT, BHMT (Betaine-homocysteine methyltransferase), BHMT1, EC 2.1.1.5, BHM0601-10 µg, BHM0601-20 µg, BHM0601-50 µg, BHM0601-100 µg, BHM0601-250 µg, BHM0601-500 µg, BHM0601-1 mg, BHM0601-010, BHM0601-020, BHM0601-050, BHM0601-100, BHM0601-250, BHM0601-500, BHM0601-01M

References & Citations

Nandita, et al., Protein Expr Purif (2002) 25:73-80; ; Breksa, A P et al., Biochemistry (1999) 38:13991-8

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001704.2

Uniprot Accession Number

Q93088

Species

Human

AA Sequence

MPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ