Recombinant human BHMT protein
Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis. Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Betaine-homocysteine S-methyltransferase 1, BHMT1, Betaine homocysteine methyltransferase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
49.2 kDa (443aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nandita, et al., Protein Expr Purif (2002) 25:73-80; ; Breksa, A P et al., Biochemistry (1999) 38:13991-8
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001704.2
Uniprot Accession Number
Q93088
Species
Human
AA Sequence
Frequently Asked Questions
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