Human Wnt-3a antibody

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors.

Product Specifications

Product Name Alternative

Wnt3a, Wingless-type MMTV integration site family, member 3A

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Wnt3a (19-352aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

3A6

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Wnt3a, AWN0924-10 µg, AWN0924-20 µg, AWN0924-50 µg, AWN0924-100 µg, AWN0924-250 µg, AWN0924-500 µg, AWN0924-1 mg, AWN0924-10, AWN0924-20, AWN0924-50, AWN0924-100, AWN0924-250, AWN0924-500, AWN0924-1

References & Citations

Pan W., et al. (2008) Science. 321 (5894) :1350-1353.; ; Katoh M., et al. (2002) Int J Oncol. 20 (2) :373-377.; ; Smolich B D., et al. (1993) Mol Biol Cell. 4 (12) :1267-1275.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Development

NCBI Accession Number

NP_149122

Uniprot Accession Number

P56704

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Wnt3a antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Wnt3a Transfected 293T cell lysate

IHC description

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)