Human visfatin/NAMPT antibody

Visfatin is pre-B cell colony-enhancing factor 1 (PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.

Product Specifications

Product Name Alternative

Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor, PBEF, PBEF1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Visfatin (1-491aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

4D5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PBEF, Visfatin, AVI0816-10 µg, AVI0816-20 µg, AVI0816-50 µg, AVI0816-100 µg, AVI0816-250 µg, AVI0816-500 µg, AVI0816-1 mg, AVI0816-10, AVI0816-20, AVI0816-50, AVI0816-100, AVI0816-250, AVI0816-500, AVI0816-1

References & Citations

Stephens JM, et al., (2006) Curr Opin Lipidol. 17:128-3.; ; Hug C, et al., (2005) Science. 307:366-367.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_005737

Uniprot Accession Number

P43490

WB Description

The cell lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: SW480 cell lysate{AVI0816-WB2.jpg}

IF Description

ICC/IF analysis of Visfatin in HeLa cells. The cell was stained with AVI0816 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .