Human Cellubrevin/VAMP-3 antibody

Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.

Product Specifications

Product Name Alternative

Vesicle-associated membrane protein 3, VAMP 3, CEB, Synaptobrevin-3, Cellubrevin, Synaptobrevin 3, VAMP3, Vesicle associated membrane protein 3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human cellubrevin (1-77aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT4G9

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cellubrevin, AVA0922-10 µg, AVA0922-20 µg, AVA0922-50 µg, AVA0922-100 µg, AVA0922-250 µg, AVA0922-500 µg, AVA0922-1 mg, AVA0922-10, AVA0922-20, AVA0922-50, AVA0922-100, AVA0922-250, AVA0922-500, AVA0922-1

References & Citations

Proux-G V., et al. (2005) Proc Natl Acad Sci USA. 102 (18) :6362-6367.; ; Randhawa Vk., et al. (2000) Mol Cell Biol. 11 (7) :2403-2417.; ; Olson AL., et al. (1997) Mol Cell Biol. 17 (5) :2425-2435.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and Flow cytometry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_004772

Uniprot Accession Number

Q15836

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Cellubrevin transfected 293T cell lysate The mouse brain tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {AVA0922-WB2.jpg}

FACS Description

Flow cytometry analysis of Cellubrevin in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .