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Human Serpin A12/Vaspin antibody

Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism.

Product Specifications

Product Name Alternative

Visceral adipose tissue-derived serine protease inhibitor, SERPINA12, Serpin peptidase inhibitor clade A member 12, Serpin A12 precursor, Serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12, OL-64, ENSG0000016595

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Vaspin (21-414aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1C4

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Visceral adipose tissue-derived serine protease inhibitor, Visceral adipose tissue derived serine protease inhibitor, SERPINA12, SERPINA 12, Serpin peptidase inhibitor clade A member 12, Serpin A12 precursor, Serpin A12, Serine proteinase inhibitor clade A member 12, Serine proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12, Serine proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12, Serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12, Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12, OL-64, OL64, OL 64, ENSG00000165953, Cysteine proteinase inhibitor clade A member 12, Cysteine proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12, Cysteine proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12, AVA0721-10 µg, AVA0721-20 µg, AVA0721-50 µg, AVA0721-100 µg, AVA0721-250 µg, AVA0721-500 µg, AVA0721-1 mg, AVA0721-01

References & Citations

Hida K , et al., (2005) Natl Acad Sci 102 (30) :10610-5; ; Klotinq N, et al., (2006) Biochem 339 (1) :430-6

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.

Scientific Category

Metabolism

NCBI Accession Number

NM_173850

Uniprot Accession Number

Q8IW75

WB Description

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Vaspin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: Vaspin transfected 293T cell lysate

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