Human VAMP-2 antibody

Vesicle-associated membrane protein 2, VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2, Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460, RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associated membrane protein 2 (synaptobrevin 2)

VAMP2, AVA0710-10 µg, AVA0710-20 µg, AVA0710-50 µg, AVA0710-100 µg, AVA0710-250 µg, AVA0710-500 µg, AVA0710-1 mg, AVA0710-10, AVA0710-20, AVA0710-50, AVA0710-100, AVA0710-250, AVA0710-500, AVA0710-1

Andres C et al., (1995) Proc Natl Acad Sci USA 92:5987-5991.; ; Jacobsson G et al., (1998) European Journal of Neuroscience 16:301-316.; ; Scales SJ. et al (2002) J Biol. Chem. 227 (31) :28271-28279.; ; Pennuto M et al., (2003) Mol Biol Cell. 14:4909-19.

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human VAMP1 protein; ; Lane 2.: Recombinant human VAMP2 protein; ; Lane 3.: Recombinant human VAMP3 protein; ; The tissue lysate (50 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain Tissue lysate{AVA0710-WB2.jpg}The tissue lysate (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Rat brain Tissue lysate{AVA0710-WB3.jpg}

ICC/IF analysis of VAMP-2 in U87MG cells. The cell was stained with AVA0710 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .