Human UNG antibody

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG) . These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species.

Product Specifications

Product Name Alternative

Uracil-DNA glycosylase isoform UNG2, uracil-DNA glycosylase, UDG, UNG1, UNG2, HIGM4, uracil-DNA glycosylase 1, uracil-DNA glycosylase 2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human UNG (1-313aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

K1C12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

UNG, AUN0713-10 µg, AUN0713-20 µg, AUN0713-50 µg, AUN0713-100 µg, AUN0713-250 µg, AUN0713-500 µg, AUN0713-1 mg, AUN0713-10, AUN0713-20, AUN0713-50, AUN0713-100, AUN0713-250, AUN0713-500, AUN0713-1

References & Citations

Jau der C et al., (1991) J Bacteriol 173 (1) :283-290.; ; Haug T et al., (1998) Nucleic Acids Res. 26 (6) :1449-1457.; ; Hilde N et al., (2000) Nucleic Acids Res. 28 (12) :2277-2285.; ; Stuart R.W. Bellamy et al., (2007) Nucleic Acids Res. 35 (5) :1478-1487.; ; Chih chung L et al., (2007) J Virol 81 (3) :1195-1208.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_550433

Uniprot Accession Number

P13051

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: K562 cell lysate; ; Lane 5.: 293T cell lysate; ; Lane 6.: HepG2 cell lysate; ; Lane 7.: A549 cell lysate; ; Lane 8.: SK-OV-3 cell lysate; ; Lane 9.: PC3 cell lysate

IF Description

ICC/IF analysis of UNG in HeLa cells. The cell was stained with AUN0713 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .